For laboratory research use only. Not for human consumption. Not for clinical or therapeutic use. Not evaluated by the FDA.
For laboratory research use only. Not for human consumption. Not for clinical or therapeutic use. Not evaluated by the FDA.
Also known as: HPLC
HPLC is the gold-standard analytical method for determining peptide purity. It separates the components of a sample so the main peak’s area can be reported as a purity percentage.
In reverse-phase HPLC, the most common mode for peptide analysis, a pressurized mobile phase carries the dissolved sample through a column packed with a hydrophobic C18 stationary phase. Individual components separate according to how strongly they interact with that phase, emerging at distinct retention times that a UV detector records as peaks on a chromatogram.
Purity is calculated by integrating the area of the main peak relative to the total area of all peaks, so a single sharp dominant peak indicates a high-purity sample while additional peaks signal impurities or degradation. This chromatogram is the primary quantitative result reported on a lot-specific certificate of analysis.